COTseq Help

Sequence alignment file

The input sequence file is an alignment of nucleotide or amino acid sequences. We accept three types of sequence files, which are fasta, nexus or phylip. The sequence data set must be in sequential format for nexus or phylip. It is recommended that the sequence name is comprised of alphabetical characters, digits or underscore. The program will replace any other characters with underscore "_".

Example for Nexus file in sequential format:

#NEXUS 

BEGIN DATA;
	DIMENSIONS  NTAX=6 NCHAR=80;
	FORMAT DATATYPE=DNA  MISSING=? GAP=- ;
MATRIX
[                      10        20        30        40        50        60        70        80   ]
[                      .         .         .         .         .         .         .         .    ]

ES9v3ed_1     ATGAAAGTGAAGCCGAGCAGGAGGAATTATCAGCACTTGTGGTGGGGCATCATGCTCCTTGGGATGTTAATGATCTGTAA   [80]
ES9v1e2_1     ATGAAAGTGAAGCCCAGCAGGAGGAATTATCAGCACTTGTGGTGGGGCATCATGCTCCTTGGGATGTTAATGATCTGTAA   [80]
ES9v1e4_1     ATGAAAGTGAAGGGGAGCAGGAGGAATTATCAGCAGTTGTGGTGAAGCATCATGCTCCTTGGGATGTTAATGATCTGTAA   [80]
ES9v1e11_1    ATGAATGTGAAGGGGAGCAGGAGGAATTATCAGCAGTTGTGGTGAAGCATCATGCTCCTTGGGATGTTAATGATCTGTAA   [80]
ES9v1e3_1     ATGAAAGTGAAGCGGAGCAGGAGGAATTATCAGCACTTGTGGTGAAGCATCATGCTCCTTGGGATGTTTTTGATCTGTAA   [80]
ES9v1e6_1     ATGAATGTGAAGGGGAGCAGGAGGAATTATCAGCACTTGTGGTGAAGCATCATGCTCCTTGGGATGTTTTTGATCTGTAA   [80]
;
END;

Example for phylip file in sequential format:

6 80
ES9v3ed_1     ATGAAAGTGAAGCCGAGCAGGAGGAATTATCAGCACTTGTGGTGGGGCATCATGCTCCTTGGGATGTTAATGATCTGTAA
ES9v1e2_1     ATGAAAGTGAAGCCCAGCAGGAGGAATTATCAGCACTTGTGGTGGGGCATCATGCTCCTTGGGATGTTAATGATCTGTAA
ES9v1e4_1     ATGAAAGTGAAGGGGAGCAGGAGGAATTATCAGCAGTTGTGGTGAAGCATCATGCTCCTTGGGATGTTAATGATCTGTAA
ES9v1e11_1    ATGAATGTGAAGGGGAGCAGGAGGAATTATCAGCAGTTGTGGTGAAGCATCATGCTCCTTGGGATGTTAATGATCTGTAA
ES9v1e3_1     ATGAAAGTGAAGCGGAGCAGGAGGAATTATCAGCACTTGTGGTGAAGCATCATGCTCCTTGGGATGTTTTTGATCTGTAA
ES9v1e6_1     ATGAATGTGAAGGGGAGCAGGAGGAATTATCAGCACTTGTGGTGAAGCATCATGCTCCTTGGGATGTTTTTGATCTGTAA

Newick tree file (optional)

A text file contains a newick tree. Users can input their own tree to reconstruct COT tree and sequence based on the input tree. If branch lengths are associated with input tree, HyPhy will ignore them and estimate branch lengths.

Phylogenetic tree output (When user-specified tree is not provided)

A newick tree estimated by PhyML under GTR (DNA) or LG (amino acid) substitution model. The tree can be viewed through the ATV Java applet.

Evolutionary parameters output (When user-specified tree is not provided)

A set of evolutionary parameters estimated by PhyML.

COT tree output

A newick tree reconstructed by HyPhy. The tree can be viewed through the ATV Java applet.

COT sequence output

COT sequence reconstructed by HyPhy under GTR (DNA) or LG (amino acid) substitution model.