The program QUALITY is a variant of the Minimum
χ2 (MC) method described by Taswell  for
limiting dilution assays, and for which he has
demonstrated by simulation desirable properties of
minimum variance (i.e., high precision) and minimum
bias. Our method modifies the MC method to allow the
user to specify the probabilities of a false negative
and false positive PCR.
The program, and the methods used, are described in
detail in Rodrigo et al. . An overview is
available in HTML and PDF
- Rodrigo, A.G., P.C.
Goracke, K. Rowhanian, and J.I. Mullins. 1997.
Quantitation of target molecules from PCR-based
limiting dilution assays. AIDS Res. Human Retrovir. 13:737-742.
- Taswell, C. 1981.
Limiting dilution assays for the determination of
immunocompetent cell frequencies. J. Immunol. 126:1614-1619.