Instructions for NetNGlycParser.pl

This program is designed to parse the output of NetNGlyc 1.0 Server that predicts N-Glycosylation sites in human proteins using artificial neural networks that examine the sequence context of Asn-Xaa-Ser/Thr sequons.

Input

  1. NetNGlyc output saved as a text file.

Output

  1. Tab delimited table lists sequence name, sequence length, total possible and over-threshold predicted N-glycosylation sites.

How to run

  1. Download NetNGlycParser.pl to your local computer, and set it as executable by typing the following command in your working directory:

    chmod +x NetNGlycParser.pl

  2. Create a subdirectory in your working directory to hold input and output files.

  3. In the subdirectory, type following command:

    perl ../NetNGlycParser.pl NetNGlycOutputFile ParseOutputFile